PCR Analysis is Sensitive and Specific for Blastocystis Infection in Jakarta Schoolchilden

Researchers in Jakarta examining 141 stool samples collected from school children report that PCR testing “revealed 89%, 78%, 80% and 88% sensitivity, specificity and positive and negative predictive values, respectively, in comparison with the culture method (McNemar, p > 0.05). Sixteen PCR samples were successfully sequenced and resulted in three Blastocystis subtypes 1, 3 and 4. In conclusion, PCR was sensitive enough and can be used to exclude Blastocystis infection up to 88% of the cases. Subtypes 3 and 1 were the main subtypes found in apparently healthy school children in Jakarta.”  The paper was published in the Journal of Tropical Pediatrics.

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